Soluble transferrin receptor stfr conversion to nmoll, mg. This study was aimed to determine the diagnostic value of serum transferrin receptor stfr and transferrin receptortransferrin index tfrf index and to combine these two markers in detecting ida in. In particular, accu reference medical lab has revolutionized testing for respiratory and gastrointestinal diseases, which allows for a significantly earlier detection of pathogens than any other technology. It may be ordered as an alternative to ferritin when you have a chronic illness andor inflammation is present or suspected. The expression of transferrin receptor tfr has been identified in many malignant tumours. Tfr are present on the surface of many cell types but they are most abundant on cells active in hemoglobin synthesis. The transferrin receptor is an integral membrane glycoprotein having a molecular weight of 190 kilodaltons kd. Review soluble transferrin receptor for the evaluation of. The significance of soluble transferrin receptors in. Soluble transferrin receptor stfr, ferritin, and stfr.
Recently, 2 different types of transferrin receptors have been recognized. Transferrin receptors, which are blood proteins, may be elevated in persons with iron deficiency. Soluble transferrin receptor stfr measurements have enabled efficient detection of early irondeficiency anemia ida, the treatment of which is essential, especially during phases of psychomotor development, rapid growth, and pregnancy. Soluble transferrin receptor and soluble transferrin. Summary of test principle and clinical relevance the method principle for measurement of soluble transferrin receptor stfr is immunoturbidimetry using roche kits on the hitachi 912 clinical analyzer. Serum transferrin receptor levels in betathalassemia trait. Role of soluble transferrin receptor and transferrin. Soluble transferrin receptor definition of soluble. Soluble transferrin receptor, human, in vitro, antibody. Soluble transferrin receptor, human, in vitro, antibody cat. Determination of serum soluble transferrin receptor stfr has been proposed to identify irondeficiency anemia ida in patients affected by concurrent inflammatory disease that.
Iron is an essential nutrient absorbed from the diet. Soluble transferrin receptor and soluble transferrin recepterferritin index for evaluation of the iron status in elderly patients. Determination of serum soluble transferrin receptor stfr is proposed to distinguish between irondeficiency anemia and. Transferrin receptor an overview sciencedirect topics. A proteolytic product of the transferrin receptor circulates in plasma as soluble transferrin receptor stfr. Soluble transferrin receptor for the evaluation of. Soluble transferrin receptor arises from proteolysis of the intact protein on the cell surface, leading to monomers that can be measured in plasma and serum. In vitro, stfr shed in the incubation medium correlates well with cellular tfr, but this relationship has never been established in vivo. Only exosomes, not plasma membranes, show the presence of a approximately 17kd peptide recognized by the antibody to the cytoplasmic domain of the transferrin receptor. Use of multiple antibiotics in patients with cf is routine. Serum transferrin receptors test reference range htq. Soluble transferrin receptor regional medical lab test. Online conversion calculator for many types of measurement units in laboratory and medicine soluble transferrin receptor stfr, stfr, transferrin receptor, tfr, serum transferrin receptors. Pdf measurement of soluble transferrin receptor in serum of.
Soluble transferrin receptors are proteins found in blood that can be elevated with iron deficiency. Soluble transferrin receptor stfr level is a marker of iron deficiency and reflects degree of availability of iron for cells. Several iron indicators can be used to detect iron deficiency anaemia ida where confounding comorbidities occurred such as patients with regular hemodialysis. Serum transferrin receptor levels for the assessment of iron status. Soluble transferrin receptor stfr test test results, normal.
Rapid onestep immunofluorometric assay for measuring soluble. Pdf soluble transferrin receptor in complicated anemia. Buy soluble transferrin receptor test at the cheapest price. Soluble and cellassociated transferrin receptor in lung. When the number of complexes decrease, the number of vacant stfrs increase. Soluble transferrin receptors and iron deficiency 347 the endosomes acidosomes, and the tftfr complex is returned back to the cell surface, where apo transferrin is released again 41. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Serum transferrin receptor stfr levels were measured with enzyme immune assay in 37 cases with. Recent studies have also suggested that stfr measurements are helpful when predicting the response to erythropoietin in patients with anaemia resultant from chronic renal failure. Serum transferrin receptor and its ratio to serum ferritin. Log ferritin index in differentiating between iron deficiency anemia. Wild, jose manuel fernandezreal, caroline hayward, ozren polasek. Serum transferrin receptor levels in betathalassemia.
Aim to determine circulating transferrin receptor levels stfr in type 2 diabetic patients to evaluate whether serum ferritin reflects iron body stores or inflammation in diabetic population. As a result, these stfrs are released into the blood stream. Hfe, also known as major histocompatibility complex mhc class ilike molecule, binds to transferrin receptor 1 tfr1 and. Human apotransferrin is a high affinity transferrin that can be used with a wide range of cells types. Serum levels of the soluble transferrin receptor stfr vary depending on the erythropoietic activity and iron status. To determine the value of serum stfr as a quantitative marker of the body mass of tissue tfr, we designed experiments to examine. Tt, in 26 patients with iron deficiency anemia ida, in 17 patients with the coexistence of. Soluble transferrin receptor and ferritin concentrations were nephelometrically determined by n latex reagent sets bade behring according to the manufacturers instructions after storage of the centrifuged 2600g.
The prevalence of iron depletion, iron deficient erythropoiesis ide, and iron deficiency anemia ida was assessed in preschool inuit children using soluble transferrin receptor stfr and traditional indicators of iron status while disregarding or taking inflammation into account when defining sf cutoffs. Soluble tfr is a truncated monomer of tissue receptor, lacking its first 100 amino acids, which circulates in the. Membranebound transferrin receptor is released externally during endocytosis. Thus, it is concluded that the truncated, soluble receptor originates from exosomes in sheep. This test measures the amount of soluble transferrin receptors within the blood, to help detect and evaluate iron deficiency and aid in the diagnosis of iron deficiency anaemia iron is an essential nutrient absorbed from the diet. Pdf soluble transferrin receptor discriminates iron. The uptake of iron by cells is mediated by a cellsurface transferrin receptor tfr. Results we found that stfr and stfrfer were significantly higher at all sampling occasions compared with early. Transferrin receptor tfr is a carrier protein for transferrin. Soluble transferrin receptor and soluble transferrin receptor. Results we found that stfr and stfrfer were significantly higher at all sampling occasions.
Soluble transferrin receptor discriminates iron deficiency anemia from anemia of chronic disease in leukemic and breast cancer patients. Recently, soluble transferrin receptor stfr levels. The serum concentration of stfr reflects the amount of the cellular receptor 1,2. Soluble transferrin receptor stfr conversion to nmoll, mgl, mgdl, mg100ml, mg%, gml. Proteolysis leads to the soluble form of the transferrin. The soluble transferrin receptor stfr test is primarily ordered to help distinguish between anemia that is caused by iron deficiency and anemia that is caused by inflammation or a chronic illness socalled anemia of chronic disease or inflammation. Soluble transferrin receptor an overview sciencedirect. It imports iron by internalizing the transferrin iron complex through receptor mediated endocytosis. On the other hand stfr levels could give an idea about erythropoietic activity in. Pdf the usefulness of soluble transferrin receptor stfr in. Each of the monomers has an 85 kd cterminal component which can bind an ironladen transferrin molecule. Hereditary hemochromatosis is an autosomal recessive disease, and 8090% of patients exhibit cys282tyr or his63asp mutations in the hfe gene.
Serum soluble transferrin receptor concentration is an. Introduction transferrin receptor tfr is a glycoprotein mediating the entry of ferric transferrin tf from the extracellular compartment into the cells. Soluble transferrin receptor is elevated in association with hyperplastic erythropoiesis, for example, in patients with haemolytic anaemia or betathalassaemia. Virtually all cells have transferrin receptors on their surface, but in the normal adult, about 80% of them are in the erythroid marrow. The stfr reflects the iron demand of the body and is postulated as a regulator of iron homeostasis via binding to the hereditary hemochromatosis protein hfe.
It aids in the diagnosis of underlying cause of anemia. Methods a total of 84 consecutive type 2 diabetic patients and 60 healthy subjects matched by age and gender were included in this case. Soluble transferrin receptor article about soluble. Development of a human soluble transferrin receptor elisa. Pdf the concentration of soluble transferrin receptor stfr in serum is reported to be useful in the diagnosis of iron deficiency, especially for. Transferrin receptor testing is used to measure the level of soluble transferrin receptors, thereby aiding in the evaluation of iron deficiency and in the diagnosis of irondeficiency anemia. As transferrin expression increases with decrease in iron concentrations,measurementofitssoluble form can be a valuable indicator of iron.
Soluble transferrin receptors and ferritin in type 2. In general, to increase sensitivity and specificity, the measurement of serum soluble transferrin receptor should be performed in combination with other tests of iron status, including ferritin, tibc, and serum iron refer to. This receptor is a protein dimer of two identical subunits, linked together by two pairs of disulfide bonds. Clinical utility of the soluble transferrin receptor and. After adding the product to the cart by clicking the button below you will see if there is a volume discount available. Data were evaluated using analysis of variance for repeated measures as the women continuing the study were found to be representative of those entering the study. Get a printable copy pdf file of the complete article 2. Serum transferrin is an independent predictor of mortality in severe alcoholic hepatitis. Order name solubl trn test number 3604710 revision date 03032009. The transferrin receptor is an essential component of cellular uptake of iron, and it binds to serum transferrin. The soluble transferrin receptor test is not routine but may be ordered along with or in follow up to other iron tests when you have a normal or nearnormal ferritin level but iron deficiency is still suspected. The serum level of stfr reflects either the cellular need for iron or the rate of erythropoiesis. A soluble form of the tfr stfr has been identified in animal and human serum. Transferrin did not correlate with markers of liver synthesis nor with nontransferrinbound iron or soluble transferrin receptor as markers of excess unbound iron and functional iron deficiency, respectively.
It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous c and nterminal domains each of which binds one ion of ferric iron. The amount of transferrin receptor expressed on a cell is proportional to the cells need for iron. Soluble interferonalpha receptor molecules are present in body fluids. Soluble transferrin receptor levels may be helpful in distinguishing iron deficiency from anemia of chronic inflammation in cf because, unlike serum ferritin and transferrin, soluble transferrin receptor does not appear to be an acutephase reactant. Soluble transferrin receptor levels are positively associated with insulin resistance but not with the metabolic syndrome or its individual components volume 116 issue 7 milton fabian suarezortegon, stela mclachlan, sarah h. Molecular structure of human transferrin transferrin. Determination of serum soluble transferrin receptor stfr has been proposed to identify irondeficiency anemia ida in patients affected by concurrent inflammatory disease that may spuriously. Shedding of transferrin receptor from rat reticulocytes during maturation in vitro.
In contrast, soluble transferrin receptor stfr is elevated in iron deficiency but is not appreciably affected by chronic disease. Study of soluble transferrin receptor and soluble transferrin receptor. Iron deficiency anemia ida and anemia of chronic disease acd are differentiated primarily by estimates of iron status iron, tibc and ferritin. Blood testing of the soluble transferrin receptor stfr is used as a measure of functional iron status and the. Accu reference medical lab is a regional leader in the fields of toxicology, pharmacogenetics and molecular testing, in addition to routine blood and urine testing. Serum soluble transferrin receptor increases in iron deficiency and is usually unaffected by chronic disease states. Soluble transferrin receptor mci diagnostic center, llc. This test is used to evaluate the level of soluble transferrin receptor in the blood. Serum transferrin is an independent predictor of mortality i. In recent years, determination of soluble transferrin receptor levels has been emerging as a test that can reliably indicate iron deficiency in various states, and that is noninvasive and easy to use. This test is ordered along with other tests including, ferritin, transferrin, total iron binding capacity, and iron test, to evaluate any kind of iron deficiency related to anemia. It is needed for the import of iron into the cell and is regulated in response to intracellular iron concentration.
It consists of two identical subunits linked by disulfide bridges. Transferrin receptor testing is used to measure the level of soluble transferrin receptors, thereby aiding in the evaluation of iron deficiency and in the diagnosis of irondeficiency anemia the reference range of transferrin receptor varies by sex in adults, as follows. If the inline pdf is not rendering correctly, you can download the pdf file here. Soluble transferrin receptor stfr and stfrlog ferritin index for. Feb 12, 2014 transferrin receptors, which are blood proteins, may be elevated in persons with iron deficiency. This bound complex then binds to soluble transferrin receptors stfrs on cells.
Role of soluble transferrin receptor and transferrin receptor. The reference range of transferrin receptor varies by. Soluble transferrin receptor test detail quest diagnostics. Levels of this receptor are increased in irondeficiency anemia. Clinical utility of the soluble transferrin receptor and comparison with serum ferritin in several populations alan e.
Serum transferrin is an independent predictor of mortality. Transferrin receptor is a nonrtk constitutively recycling receptor and thus can be used as a control to assess rtk specificity of met trafficking. The human transferrin receptor tfr is shed by an integral metalloprotease releasing a soluble form stfr into serum. The soluble transferrin receptor stfr has been introduced as a promising new diagnostic tool for differentiating between iron deficiency anemia ida and anem we use cookies to enhance your experience on our website. Measurement of circulating levels of the receptor are very useful in the diagnosis of iron deficiency in patients who also have anemia. Origin of a soluble truncated transferrin receptor blood. In lung cancer, lymphoma and breast cancer, it has been shown that the expression of tfr correlates with tumour differentiation, probably implying some prognostic value.
The transferrin receptor is comprised of two identical subunits, each composed of 760 aminoacids with a molecular mass of 95 kdaltons. Soluble transferrin receptor levels in plasma are elevated if there is increased iron demand due to iron deficiency, increased erythropoiesis eg, haemolysis or dyserythropoiesis eg, megaloblastic anaemia, regardless of other, coexistent states. Elevated stfr values are found in anemia of chronic disease acd, iron deficiency anemia ida, polycythemia, hemolytic anemia, thalassemia, hereditary spherocytosis, sickle cell and megaloblastic anemia, myelodysplastic syndrome and vitamin b12 deficiency. Soluble transferrin receptor an overview sciencedirect topics. Soluble transferrin receptor assay stfr may be useful in these situations because it reflects the degree of iron requirement in relation to supply, and it is not an acute phase reactant. Enhancement of interleukin 6 cytostatic effect on human breast carcinoma cells by soluble il6 receptor from urine and reversion by monoclonal antibody. Pdf we evaluated the usefulness of soluble transferrin receptor stfr and of the stfrlog ferritin indexstfrlogf in the differentiation of anemia. It is usually not affected by chronic disease states, stfr levels are about 6% higher in people in high altitudes above 5200 ft1600 m and in africanamericans. Soluble tfr is a truncated monomer of tissue receptor, lacking its first 100 amino. Detection of soluble hfe associated with soluble transferrin. Soluble transferrin receptor conventionally refers to the cleaved extracellular portion of transferrin receptor 1 that is released into serum.
Soluble transferrin receptors and iron deficiency, a step. The existence of a receptor for transferrin iron uptake had been recognized over half a century back. Soluble transferrin receptor stfr is a circulating, cleaved form of a membrane receptor protein. Using soluble transferrin receptor and taking inflammation. Release of the soluble transferrin receptor is directly. This gene encodes a glycoprotein with an approximate molecular weight of 76. Serum transferrin receptor and its ratio to serum ferritin in. This test measures the amount of soluble transferrin receptors to help detect and evaluate iron deficiency and aid in the diagnosis of iron deficiency anemia iron is an essential nutrient absorbed from the diet. It imports iron by internalizing the transferriniron complex through receptormediated endocytosis. Furthermore, the distinction between ida and anemia. Soluble and cellassociated transferrin receptor in lung cancer. Rapid onestep immunofluorometric assay for measuring. Thus, the concentration of stfr in plasma or serum is an indirect measure of total tfr. This test measures the amount of soluble transferrin receptors within the blood, to help detect and evaluate iron deficiency and aid in the diagnosis of iron deficiency anaemia.
Soluble transferrin receptor arup lab test directory. Measurement of soluble transferrin receptor in serum of. By continuing to use our website, you are agreeing to our use of cookies. Patients with sah had diminished serum transferrin but increased transferrin saturation.
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